Table 2

Detection of lacZ- phage
Packaging exp.
 RAd
 Genotype
 Animal number
 Packaging
 Total number of plaque formers
 lacZ- plaques
 Mutant Frequency
 Expected genotype
1
 None
 Not relevant
 #0
 Tube 1
 4.5 × 104
 4
 8.9 × 10-5
 n.t.




Tube 2
 3.2 × 104
 3
 9.4 × 10-5
 n.t.




Tube 3
 8.5 × 104
 8
 9.4 × 10-5
 n.t.











average 9.2 × 10-5


AdNY56
 lacZ+
 #1
 Tube 4
 8.5 × 104
 8
 9.4 × 10-5
 n.t.




Tube 5
 6.4 × 104
 3
 4.7 × 10-5
 n.t.




Tube 6
 8.8 × 104
 1
 1.1 × 10-5
 n.t.











average 5.1 × 10-5
2
 None
 Not relevant
 #0
 Tube 7
 4.9 × 104
 5
 10 × 10-5
 n.t.




Tube 8
 6.6 × 104
 4
 6.1 × 10-5
 n.t.




Tube 9
 5.1 × 104
 10
 20 × 10-5
 n.t.











average 12 × 10-5


AdNY57
 lacZ- (Glu461Gly)
 #2
 Tube 10
 3.8 × 104
 6
 16 × 10-5
 0/6




Tube 11
 3.0 × 104
 7
 23 × 10-5
 0/7




Tube 12
 4.5 × 104
 9
 20 × 10-5
 0/9











average 20 × 10-5
 total 0/22
3
 None
 Not relevant
 #0
 Tube 13
 3.7 × 104
 6
 16 × 10-5
 n.t.




Tube 14
 6.0 × 104
 5
 8.3 × 10-5
 n.t.




Tube 15
 4.4 × 104
 4
 9.1 × 10-5
 n.t.











average 11 × 10-5


AdNY57
 lacZ- (Glu461Gly)
 #2
 Tube 16
 1.3 × 104
 9
 69 × 10-5
 0/9




Tube 17
 3.9 × 104
 19
 49 × 10-5
 0/19




Tube 18
 6.5 × 104
 26
 40 × 10-5
 0/26











average 53 × 10-5
 total 0/54
4
 None
 Not relevant
 #0
 Tube 19
 2.6 × 105
 8
 8.5 × 10-5
 n.t.


AdNY57
 lacZ- (Glu461Gly)
 #3
 Tube 20
 1.6 × 105
 5
 6.3 × 10-5
 0/5




Tube 21
 4.1 × 105
 9
 8.6 × 10-5
 0/9











average 1.5 × 10-5
 total 0/14
5
 None
 Not relevant
 #0
 Tube 22
 3.3 × 104
 3
 9.1 × 10-5
 n.t.


AdNY58
 lacZ- (Tyr105 Stop)
 #4
 Tube 23
 8.6 × 104
 4
 4.7 × 10-5
 0/4




Tube 24
 3.1 × 104
 3
 9.7 × 10-5
 0/3











average 7.2 × 10-5
 total 0/7

RAd: Recombinant adenovirus

n.t.: Not tested.

Ino et al. Genetic Vaccines and Therapy 2005 3:8   doi:10.1186/1479-0556-3-8