Figure 3.

Increased gene expression with scAAV after longer intervals. CD-1 mice (Charles Rivers) injected 4 days after birth with 1.85 × 10¹¹ vector genomes (vg) of either scAAV or ssAAV using sub-xiphoid injections [14]. The animals were returned to their dams for 11 days (A) or 21 days (B) and then processed as described in Figure 2. Representative sections with GFP fluorescence are pictured. The bar represents 150 μm in the 21 day ssAAV-apex and 300 μm in all other panels.