Careful adjustment of Epo non-viral gene therapy for β-thalassemic anaemia treatment

doi:10.1186/1479-0556-6-10

Genetic Vaccines and Therapy

Volume 6

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Fabre EE
Bigey P
Beuzard Y
Scherman D
Payen E

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Bigey P
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Scherman D
Payen E
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Careful adjustment of Epo non-viral gene therapy for β-thalassemic anaemia treatment

Emmanuelle E Fabre¹^,2^,3^,4 , Pascal Bigey¹^,2^,3^,4 , Yves Beuzard⁵ , Daniel Scherman¹^,2^,3^,4 and Emmanuel Payen⁵

¹Unité de Pharmacologie Chimique et Génétique, INSERM U640, Faculté de Pharmacie, 4 avenue de l'observatoire, 75006 Paris, France

²Unité de Pharmacologie Chimique et Génétique, CNRS UMR 8151, Faculté de Pharmacie, 4 avenue de l'observatoire, 75006 Paris, France

³Unité de Pharmacologie Chimique et Génétique, Université Paris Descartes, Faculté de Pharmacie, 4 avenue de l'observatoire, 75006 Paris, France

⁴Unité de Pharmacologie Chimique et Génétique, Ecole Nationale Supérieure de Chimie de Paris, 11 rue Pierre et Marie Curie, 75005 Paris, France

⁵Laboratoire de Thérapie Génique Hématopoïétique, Institut d'Hématologie (IUH), INSERM U733, Hôpital Saint-Louis, 75011 Paris, France

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Genetic Vaccines and Therapy 2008, 6:10doi:10.1186/1479-0556-6-10


Published:	11 March 2008

Additional files

Additional file 1:

Changes in erythropoietin (Epo) levels after repeated muscular electrotransfer of 1 μg and 1.5 μg of Epo-plasmid. the data provided shows the mean EPO level reached in mice following the electrotransfer treatments, for all three groups of mice (ie, control group, 1 μg treated group and 1.5 μg treated group). Mouse Epo changes in β-thalassemic mice electrotransfered with NaCl 150 mM solution for control group (solid diamond) or with 1 μg (solid sphere) and 1.5 μg (solid square) Epo-plasmid doses for the other groups. Electrotransfer was performed at day 0, 34, 112 and 215 for the three groups. One additional electrotransfer was performed at day 77 for the 1 μg group. Arrows indicate electrotransfer applications. The EPO ELISA Medac^™kit was used to measure mouse Epo based on cross-reaction (detection limit of 25 mU/ml for human Epo). Data are presented as mean Epo levels with standard error of the mean (SEM).

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