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Careful adjustment of Epo non-viral gene therapy for β-thalassemic anaemia treatment

Emmanuelle E Fabre1,2,3,4 email, Pascal Bigey1,2,3,4 email, Yves Beuzard5 email, Daniel Scherman1,2,3,4 email and Emmanuel Payen5 email

1Unité de Pharmacologie Chimique et Génétique, INSERM U640, Faculté de Pharmacie, 4 avenue de l'observatoire, 75006 Paris, France

2Unité de Pharmacologie Chimique et Génétique, CNRS UMR 8151, Faculté de Pharmacie, 4 avenue de l'observatoire, 75006 Paris, France

3Unité de Pharmacologie Chimique et Génétique, Université Paris Descartes, Faculté de Pharmacie, 4 avenue de l'observatoire, 75006 Paris, France

4Unité de Pharmacologie Chimique et Génétique, Ecole Nationale Supérieure de Chimie de Paris, 11 rue Pierre et Marie Curie, 75005 Paris, France

5Laboratoire de Thérapie Génique Hématopoïétique, Institut d'Hématologie (IUH), INSERM U733, Hôpital Saint-Louis, 75011 Paris, France

author email corresponding author email

Genetic Vaccines and Therapy 2008, 6:10doi:10.1186/1479-0556-6-10

Published: 11 March 2008

Additional files

Additional file 1:

Changes in erythropoietin (Epo) levels after repeated muscular electrotransfer of 1 μg and 1.5 μg of Epo-plasmid. the data provided shows the mean EPO level reached in mice following the electrotransfer treatments, for all three groups of mice (ie, control group, 1 μg treated group and 1.5 μg treated group). Mouse Epo changes in β-thalassemic mice electrotransfered with NaCl 150 mM solution for control group (solid diamond) or with 1 μg (solid sphere) and 1.5 μg (solid square) Epo-plasmid doses for the other groups. Electrotransfer was performed at day 0, 34, 112 and 215 for the three groups. One additional electrotransfer was performed at day 77 for the 1 μg group. Arrows indicate electrotransfer applications. The EPO ELISA Medackit was used to measure mouse Epo based on cross-reaction (detection limit of 25 mU/ml for human Epo). Data are presented as mean Epo levels with standard error of the mean (SEM).

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