Figure 2.

Levels of pDNAX detected by QRTPCR in calf muscle (at the injection site) after administration of 10 μg pDNAX in 8-week old BalB/C mice. The line connects the average levels of plasmid DNA detected by QRT-PCR in 500 ng of isolated DNA (MC/r) ± SD (four mice per time point). The straight line represents quantification limit of QRTPCR assay (10 pDNAX copies/reaction). The dotted line represent detection limit of QRTPCR assay (3 pDNAX copies/reaction). The data from control group were omitted (all control animals were negative). Routes of application: full circle denotes naked pDNAX; full triangle denotes pDNAX plus electroporation; full square denotes pDNAX:CDAN-DOPE complex.