|
Effect of various pDNAX formulations on its persistence in beef cattle after i.m. administration |
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| Beef cattle groups |
Beef cattle ID code |
Interval between 2nd immunisation and slaughter (days) |
pDNA copies at the injection site/500 μg DNA (n = 5) |
pDNA copies opposite -to- injection site muscle (n = 4) |
pDNA copies distant muscle (n = 3) |
pDNA copies DLNa total (n = 6) |
|
|
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| pDNA |
20087 |
242 |
< LQL (2); < DL (2); Neg. (1) |
0/4 |
0/3 |
0/6 |
| 20105 |
277 |
73.97; 35.59; < LQL (2); < DL (1) |
0/4 |
0/3 |
0/6 |
|
| 20080 |
284 |
< LQL (5) |
0/4 |
0/3 |
0/6 |
|
|
|
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| DNA + B30-Nor-AbuMDP |
20083 |
242 |
19.86; 16.23; 15.5; < LQL (2) |
0/4 |
0/3 |
0/6 |
| 28504 |
270 |
92.78; 29.25; 28;92; 24.04; 23.75 |
0/4 |
0/3 |
0/6 |
|
| 20090 |
291 |
13.48; 12.73; 10.87; < LQL (1); Neg. (1) |
< DL(1/4) |
0/3 |
0/6 |
|
|
|
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| DNA:cationic liposome complex |
20086 |
270 |
288; 220.08; 200.60; 30.07; < LQL (1) |
0/4 |
0/3 |
0/6 |
| 20089 |
277 |
228.9; 169.90, 134.70; 39.57; 39.00 |
0/4 |
0/3 |
0/6 |
|
| 3654 |
291 |
149.3; 64.79; 46.60; 18.88; 18.73 |
0/4 |
0/3 |
0/6 |
|
| 20081 |
298 |
< LQL (5) |
0/4 |
0/3 |
0/6 |
|
|
The total amount 1000 μg of pDNAX in two equal doses was delivered into coccygeus muscle (injection site) as naked pDNAX, naked pDNAX + liposomal B30-norAbuMDP, and cationic liposome complex pDNAX:CDAN-DOPE. The level of pDNAX in the injection site, opposite-to-injection site, distant muscle tissues and draining lymph nodes was examined after 242–298 days after the second immunisation. Plasmid copies are expressed as mean plasmid copies per 500 ng of genomic DNA (MC/r) from duplicate QRTPCR assay. < LQL: below linear limit of quantification (10 copies/reaction), < DL: below detection limit (3 copies/reaction), Neg.: negative sample, aDLN- draining lymph nodes. | ||||||
Orság et al. Genetic Vaccines and Therapy 2008 6:11 doi:10.1186/1479-0556-6-11 |
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