|
Observed amino acid sequences of AAV capsid mutant as observed during four screening rounds on K562 cells. |
|||
| Selection round |
|||
|
|
|||
| Insert sequence |
2nd |
3rd |
4th |
|
|
|||
| GISPRAG |
2 |
2 |
→ |
| GVSGRPA |
2 |
2 |
→ |
| NESRVLS |
2 |
2 |
2. |
| KDPVRAP |
→ |
2 |
3. |
| ASRPP |
5 |
2 |
3. |
| EARVRPP |
→ |
→ |
4. |
| NSVSLYT |
→ |
→ |
5. |
| Others |
8 |
7 |
1. |
|
|
|||
| Total |
19 |
17 |
18 |
|
The bolded clones EARVRPP and NSVSLYT were chosen for further investigation. → clone not detected. | |||
Stiefelhagen et al. Genetic Vaccines and Therapy 2008 6:12 doi:10.1186/1479-0556-6-12 |
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