Table 2

Gene transfer efficiency of K562-targeted and standard rAAV2 vectors on solid tumour cells.


%GFP+ cells
MFI (AU)

Cell line
EARVRPP
rAAV2
EARVRPP
rAAV2

RM-HS-1
46.01 ± 10.82
97.61 ± 3.21
35.03 ± 12.93
395.64 ± 52.93
H-Meso1
76.42 ± 2.41
88.30 ± 4.34
70.29 ± 9.54
150.89 ± 23.22
HeLa-RC
77.94 ± 6.60
98.22 ± 1.21
32.57 ± 7.73
143.49 ± 12.21

A significant (p < 0.05; irrespective of cell line) superiority of the standard rAAV2 vector on three solid tumour cell lines could be shown for both %GFP+ cells and MFI. MFI: mean fluorescence intensity; AU: Arbitrary units.

Stiefelhagen et al. Genetic Vaccines and Therapy 2008 6:12   doi:10.1186/1479-0556-6-12