Figure 1.

PSA antigen expression of immature DCs transfected with total RNA from LNCaP cells. Immature DCs were transfected by electroporation with 4 μg (IA and IB) and 16 μg (IIA and IIB) of RNA from LNCaP cells. The electroporation conditions were 300 (IA and IIA) or 400 V (IB and IIB). In both cases, the capacitance was 25 μF. IIIA is the negative transfection control, and IIIB is the positive control represented by LNCaP. The panel of photographs represents PSA expression detected by immunocytochemistry using an anti-PSA antibody.