Figure 4.

Cytokine production in BALB/c mice is altered by siNPRA-imiquimod treatment. (A) IL-4 in BAL fluid was measured by IL-4 ELISA. Significant reduction of IL-4 was achieved by siNPRA-imiquimod treatment compared to OVA controls. (B) Lungs of all animals from the four groups were removed and homogenized. The levels of IL-2, IL-5, IFN-γ and TNFα in lung homogenates were measured using a mouse Th1/Th2 Cytokine CBA kit. IL-5 was significantly downregulated by siNPRA treatment. Results shown are averages of two separate experiments with standard deviations (*, P < 0.05, **, P < 0.01).