Table 3

The protective effect of vaccination with naked DNA or liposome formulated DNA.

Vaccine group

Brucella CFU per spleen

Brucella per spleen as a % of challenge dose

Protection units

Experiment 1: A comparison of single dose naked DNA vaccine efficacy

Rev.1

2.95 ± 0.35*

67.8

2.04*

PBS

5.00 ± 0.05

114.8

0

pcDNA3.1 [X1]

4.56 ± 0.31

104.8

0.43

p-omp25 [X1]

4.45 ± 0.44

102.3

0.55

p-ialB [X1]

4.19 ± 0.42

96.4

0.80

Experiment 2: p-omp25 [X4] compared with L-p-omp25 [X1]

Rev.1

1.47 ± 0.82*

33.2

3.35*

PBS

4.83 ± 0.29

109.2

0

pcDNA3.1 [X4]

4.80 ± 0.21

108.5

0.02

p-omp25 [X4]

1.40 ± 0.69*

31.7

3.42*

L-p-omp25 [X1]

4.01 ± 0.14*

90.8

0.81*

L-pcDNA3.1

4.86 ± 0.44

109.8

-0.03

Experiment 3: p-ialB [X4] compared with L-p-ialB

Rev.1

1.94 ± 0.77*

44.8

2.12*

PBS [X4]

4.10 ± 0.68

93.6

0

PcDNA3.1 [X4]

3.75 ± 0.36

86.1

0.32

p-ialB [X4]

1.91 ± 0.93*

44.0

2.15*

L-p-ialB [X1]

3.07 ± 1.08

70.5

1.00

L-pcDNA3.1

3.37 ± 0.58

77.5

0.70

Brucella CFU per spleen: Log Brucella CFU per spleen ± standard deviation.

Protection units = Log Brucella CFU per spleen of unvaccinated mice - Log Brucella CFU per spleen of vaccinated mice. % Challenge dose: (Log CFU Brucella per spleen/Log CFU challenge dose) × 100.

* indicates statistically significant reduction of Brucella CFU per spleen compared to PBS controls in the same study (One Way ANOVA analysis with Dunnets post-hoc test, p < 0.05)

Commander et al. Genetic Vaccines and Therapy 2010 8:5   doi:10.1186/1479-0556-8-5