|
Comparison of various methods in the transfection efficiency of leukemic cells. Transfections were performed with an expression plasmid for eGFP or adenoviral GFP expressing vector as described in materials and methods. 24 hours after transfections cells were harvested and assayed by flow cytometry. Ad-GFP transfected cells were analyzed 72 hours after transduction. Results of three separate experiments are presented (ND, not done). |
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| Cell line |
Adenoviral gene transfer (MOI 200) GFP-pos. cells [%] |
Electroporation GFP-pos. cells [%] |
Gene gun GFP-pos. cells [%] |
Nucleofection GFP-pos. cells [%] |
|
|
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| primary AML cells |
ND |
ND |
ND |
60.3 +/- 9.7 |
| K562 |
49 +/- 4 |
15.5 +/- 3.5 |
1.5 +/- 0.5 |
74.7 +/- 8.0 |
| HL60 |
ND |
30.2 +/- 5.6 |
3.0 +/- 1 |
49.0 +/- 9.7 |
Schakowski et al. Genetic Vaccines and Therapy 2004 2:1 doi:10.1186/1479-0556-2-1 |
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