Genetic Vaccines and Therapy

unofficial impact factor 1.86
Search for
Advanced search
Genetic Vaccines and Therapy
Tools
Open Access Research

Sustained protective rabies neutralizing antibody titers after administration of cationic lipid-formulated pDNA vaccine

Michal Margalith and Adrián Vilalta*

Author Affiliations

Vical Incorporated, 10390 Pacific Center Ct, San Diego, CA 92121, USA

For all author emails, please log on.

Genetic Vaccines and Therapy 2006, 4:2 doi:10.1186/1479-0556-4-2

Published: 15 February 2006

Abstract

Published data indicate that formulation of pDNA with cationic lipids could greatly enhance the response to a pDNA vaccine in larger mammals. The present work tested the influence of several pDNA:cationic lipid formulations on rabies neutralizing titers. Plasmid expressing Rabies G protein (CVS strain) was evaluated in vivo for ability to elicit neutralizing titers. pDNA:DMRIE-DOPE formulated at two DNA:cationic lipid molar ratios was compared in mice to a Vaxfectin™-pDNA formulation. Mouse data indicate that Vaxfectin™ is more effective than DMRIE-DOPE in eliciting neutralizing titers. In addition, the ratio of pDNA to DMRIE-DOPE can also affect neutralizing titers. Our data show that sustained neutralizing titers (120 days) can be obtained after a single administration of DMRIE-DOPE-formulated pDNA in rabbits.