Figure 3.

Histological analysis of human FIX expression in livers of AAV8-LP1-hFIX infused rats. Representative liver sections of immunohistochemical staining for hFIX 4 months after gene transfer in rats infused with scAAV8-LP1-hFIX at (A) 5 × 10¹¹vg/rat, (B) 1 × 10¹¹vg/rat, (C) 5 × 10¹⁰vg/rat, (D) 1 × 10¹⁰vg/rat or (E) saline. Paraffin liver sections were stained for human FIX expression using goat anti-human FIX conjugated to horse-radish peroxidase and goat IgG was used as the negative control (F). Magnification is × 40.